FRCR MCQ---MYOCARDIAL PERFUSION/REPORTER GENE IMAGING

19.Myocardial perfusion scan uses 

a. ²⁰¹Tl (thallous chloride)--- K+ analogue indicating perfusion (ischaemic heart disease) (delayed uptake reflects viability)

b. ^99mTc isonitriles---Cationic complexes taken up by myocytes in proportion to blood flow

c. ^99mTc teboroxime---Lipophilic compound which accumulates by diffusion

d. ^99mTc phosphines----Uptake proportional to blood flow

e. ^99mTc red blood cells---Blood pool label

 ANS-----

35.True regarding  reporter gene –substrate combination

a. the herpes simplex virus type 1 thymidine kinase (HSV1-TK) and  fluoro-deoxy-arabinofuranosyl-iodouracil or FIAU

b. genes encoding for a modified human transferrin receptor and supraparamagnetic iron compounds

c. β-galactosidase and gadolinium contrast agent (EgadMe)

d. the herpes simplex virus type 1 thymidine kinase (HSV1-TK) and  (EgadMe)

e.  the herpes simplex virus type 1 thymidine kinase (HSV1-TK)  and gadolinium contrast agent (EgadMe)

35.abc 

REPORTER GENE IMAGING

 In reporter gene imaging,the reporter gene is incorporated into the nuclear DNA of the target cells by transfection (e.g. viral vector.The activated reporter gene is transcribed into the corresponding messenger ribonucleic acid (mRNA).Messenger RNA (mRNA)  is translated to the reporter protein.A reporter substrate  is admninistered.The reporter substrate interacts with the reporter protein to produce an imaging signal (e.g. gamma ray, paramagnetic effect).